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Registros recuperados: 34 | |
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Lázzari,Sonia M. N.. |
Seventeen clones of Rhopalosiphum padi (Linnaeus, 1758), 14 of R. maidis (Fitch, 1856), and two of R. insertum (Walker, 1849), representing a wide range of host plants and geographic distribution, were examined electrophoretically to determine intra and interspecific variation. Twenty-one enzyme systems were tested using starch-gel techniques. The electromorph variation within species was low, as expected for parthenogenetic organisms. Frequency of heterozygotes was also relatively low for most populations. The percentage of polymorphic loci ranged from 0% to 27.3% in R. padi, but it was lower (0% to 18.2%) in the completely an holocyclic R. maidis. No consistent correlation between band patterns and host plant or geographic origin could be established for... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rhopalosiphum; Aphididae; Homoptera; Isozymes. |
Ano: 1992 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751992000100015 |
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SANTOS,Jaselita M.M. dos; TADEI,Wanderli Pedro; CONTEL,Eucléia P.B. |
Of eleven proteins analyzed in four Amazonian populations, the esterases showed the greatest variation, with five activity zones. EST1, EST2 and EST5 showed variation in each of the populations studied. EST1 and EST2 are each controlled by two, and EST5 by four, codomi-nant alleles. LAP presented six activity zones, with codominant variation in LAP5and LAP6.oc—GPDH was monomorphic with one activity band on starch gel and two on polyacrylamide gel. 1DH presented two activity zones, with variation in the IDHl region. PGM had a single activity zone, with variation in all populations. The Ariquemes populations showed five alleles and the other populations three, all of then codominant. Three activity zones with two codominant alleles were observed for ODH.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anopheles darlingi; Isozymes; Electrophoresis. |
Ano: 1996 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59671996000200097 |
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TELLES,MARIANA P. DE C.; DINIZ-FILHO,JOSÉ ALEXANDRE F.; COELHO,ALEXANDRE S.G.; CHAVES,LÁZARO J.. |
Dentre as diversas árvores frutíferas nativas dos cerrados, a cagaiteira (Eugenia dysenterica DC.) merece destaque pelo seu amplo potencial econômico. A fim de fornecer algumas informações relativas ao padrão espacial da variabilidade genética desta espécie, foram realizadas análises de autocorrelação espacial das freqüências alélicas em dez subpopulações locais da região sudeste do Estado de Goiás. Foram utilizados marcadores isoenzimáticos, em um total de seis sistemas enzimáticos (SKDH, 6-PGD, alfa-EST, MDH, PGI e PGM), com oito locos polimórficos. Foi realizada uma análise de autocorrelação espacial, utilizando índices I de Moran estimados em quatro classes de distância geográfica. Os correlogramas mostraram que, de fato, a divergência genética está... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eugenia dysenterica; Isozymes; Neutral evolution; Spatial autocorrelation. |
Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000200003 |
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ENÉAS-FILHO,JOAQUIM; BARBOSA,GISLAINY KARLA DA COSTA; SUDÉRIO,FABRÍCIO BONFIM; PRISCO,JOSÉ TARQUÍNIO; GOMES-FILHO,ENÉAS. |
Three isoforms of beta-galactosidases were isolated and partially purified from the cotyledons of quiescent seeds of Vita 3 and Vita 5 cowpea [Vigna unguiculata (L.) Walp.] cultivars differing in water and salt stress tolerance. The purification procedure consisted of ammonium sulfate fractionation, acid precipitation, ion exchange chromatography through DEAE-sephadex and affinity chromatography through Lactosyl-sepharose columns. The three isoforms isolated from the two cultivars showed the same chromatographic patterns, same optimum of temperature for enzyme activity assay (60ºC), identical thermal stability up to 50°C, and similar pH optima (3-4). However, they differed from each other in sensitivity towards metal ions and certain chemical agents... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cotyledons; Isozymes; Vigna unguiculata; Vita 3 and Vita 5 cultivars; Salt tolerance. |
Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000300001 |
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OLIVEIRA,ALESSANDRO F.; CARVALHO,DULCINÉIA DE; ROSADO,SEBASTIÃO C.S.. |
Uma população natural de Copaifera langsdorffii Desf., espécie arbórea comumente encontrada no Brasil, foi estudada por meio da eletroforese de isoenzimas, visando determinar a taxa de cruzamento e o sistema reprodutivo. A população amostrada localiza-se ao longo de um trecho do rio Capivari, em uma área de preservação permanente (floresta ciliar), entre os municípios de Lavras e Itumirim, sul do Estado de Minas Gerais, Brasil. Foram amostrados tecidos foliares de 20 indivíduos adultos da população e 400 indivíduos jovens (progênies) procedentes de sementes coletadas de 20 matrizes na população. Cinco sistemas enzimáticos revelaram 24 e 29 alelos totais para os indivíduos adultos e progênies, distribuídos em 12 locos. O sistema reprodutivo desta população... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Copaifera langsdorffii; Isozymes; Hardy-Weinberg Equilibrium. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042002000300009 |
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Cabral,Betânia Lúcia Rocha; Souza,Joelson Araújo de; Ando,Akihiko; Veasey,Elizabeth Ann; Cardoso,Eloisa Maria Ramos. |
Cassava (Manihot esculenta Crantz) belongs to the Euphorbiaceae family, and is widely cultivated in Brazil. The objective of this study was to evaluate the isoenzymatic variability of 200 cassava accessions from the germplasm bank of Embrapa Amazonia Oriental. Seven groups were formed according to their origin: 1-Amazonas, 2-Amapa, 3-Bahia, 4-Para, 5-Rondonia, 6-Various, for accessions with a maximum of three individuals per place of origin, and 7 - Accessions of indefinite origin. The accessions were also evaluated as a whole. For the electrophoretic analyses, samples of young leaves were used in a 12% starch gel. Eight isoenzymatic systems were evaluated: acid phosphatase (ACP), leucine aminopeptidase (LAP), glucose-6-phosphate dehydrogenase (G6PDH),... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Manihot esculenta; Genetic variability; Germplasm bank; Isozymes. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000300017 |
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Collares,Eliana Antonia Silveira; Choer,Eva; Pereira,Arione da Silva. |
The objective of this work was to characterize 27 potato genotypes, using molecular markers. Polyacrylamide gel electrophoresis, RAPD techniques and isozymes of esterase, phosphoglucomutase and soluble proteins were analyzed in tubers, and isocitrate dehydrogenase, aspartate transaminase, phosphoglucomutase and peroxidase, in leaves. Eighteen primers were tested and four were chosen, kits OPX (01, 04 and 09) and OPY (07), to analyze RAPD markers in leaf extracts. Similarity and cluster analysis were conducted using Jaccard coefficient and the unweighted pair-group method using arithmetic average. Despite the differences detected in the analysis of proteins and isozymes in the tubers, as well as of isozymes in the leaves, the characterization of all... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Solanum tuberosum; Electrophoresis; Isozymes; RAPD; Genetic similarity. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000900006 |
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Lozoya-Saldaña,Héctor; Guzmán-Galindo,Leonel; Fernández-Pavia,Sylvia; Grünwald,Niklaus J.; McElhinny,Elaine. |
Abstract The molecular identity of Phytophthora infestans strains infecting potatoes in the Toluca Valley has been documented. Nevertheless, the relationship of the P. infestans genotypes to the host’s genetic resistance is unclear. In order to identify potential host-pathogen specificity, isolates of the oomycete obtained from five potato varieties with differing levels of disease resistance were compared regarding mating type (MT), isozyme genotype for glucose-6-phosphate isomerase (Gpi) and peptidase (Pep), and sensitivity to metalaxyl. Also, host resistance components and specificity on the host-pathogen interaction were assessed twice in detached leaves in the laboratory and once in attached leaves in the greenhouse for three of the varieties. No... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Compatibility; Isozymes; Metalaxyl resistance; Genetic resistance; Host-pathogen specificity. |
Ano: 2006 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952006000200205 |
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González González, Miguel. |
Para diseñar estrategias de conservación in situ de la diversidad genética del maíz, se requiere de valorar tanto a la diversidad como al potencial para incrementar la producción bajo el esquema de la agricultura tradicional como punto de referencia. Dado que el intercambio de semilla se da principalmente dentro de las comunidades rurales, se consideró al municipio de Ayapango, Edo. de Méx., para colectar las poblaciones de maíz que se manejan en aproximadamente el 20% de los hogares. Se colectaron 97 poblaciones de la raza Chalqueño, y dos de Cacahuacintle. Dentro de Chalqueño hubo poblaciones del tipo de grano cremoso (49), del conocido localmente como Palomo (19), del azul (24), del rojo (4) denominado localmente como “xitocle”, y una población de... |
Tipo: Tesis |
Palavras-chave: Diversidad genética; Maíz; Chalqueño; Conservación in situ; Isoenzimas; Doctorado; Genética; Genetic diversity; Maize; Chalqueño; In situ conservation; Isozymes. |
Ano: 2007 |
URL: http://hdl.handle.net/10521/1585 |
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Cinto,Isabel E.; Dokmetzian,Diana A.; Ranalli,María E.. |
The aim of this study was to delimit two Iodophanus species: I. carneus and I. testaceus , based on morphological characteristics and electrophoretic patterns of their intracellular isozymes. Twenty monosporic strains were used, including five belonging to I. granulipolaris as a control. Fourteen isozyme systems were tested, and the five having the best resolution selected: aspartate aminotransferase, esterases, alkaline phosphatase, glutamate dehydrogenase, and superoxide dismutase. These analyses confirmed the similarity between I. carneus and I. testaceus , since they both produced the same band patterns, which were in turn different from the band pattern of I. granulipolaris. So, as we couldn´t find any character wich permit us to classify the isolated... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fungi; Lodophanus; Isozymes; Synonymy; Taxonomy. |
Ano: 2007 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722007000200005 |
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Registros recuperados: 34 | |
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